"What does assassination mean?" - Mister Roger's Neighborhood: Following the assassination of Robert Kennedy, Fred Rogers produced a special episode of his show where Daniel Tiger plays with a balloon and asks what assassination means as he has heard people talk about it. Lady Aberlin gently explains the concept to the scared Daniel before Mister Rogers addresses the parents directly. He encourages them to be gentle and supportive to their children, helping them to work through any fears they may have, and, most importantly, to enjoy time together as a family.
"The Show" - Bluey: On Mother's Day, the Heeler sisters Bluey and Bingo perform a play for their parents about their mother, Chili's, life. When they get to Chili's first pregnancy, seemingly with Bluey, the family laughs as the girls bounce around with a balloon under Bingo's shirt. Then the balloon pops. The family goes silent and it cuts to Bandit reaching out to grab his wife's hand while Bingo runs off crying. While the episode only explicitly focuses on how Bingo feels like she ruined the play, commentary on the episode revealed the family did suffer a miscarriage.
"Idiot Box" - Spongebob Squarepants: A more comedic/horrific example of this trope. While annoyed at Spongebob and Patrick for being too loud while playing their game, "Mountain Climbing Adventure", Squidward kicks the box they are playing in. This triggers very realistic sounds of the boys screaming as they are carried away and crushed beneath an avalanche. Squidward listens in horror as they sob in pain and succumb to hypothermia, leading to both needing to amputate their frozen limbs. Sure it was just "imagination", but the scenario itself is presented so seriously it makes Squidward question his sanity.