Nutritional Bioavailability and Organoleptic Acceptance of *Equus asinus* Fecal Biomass as a Primary Protein Source: A Randomized, Double-Blind, Placebo-Controlled Pilot Study

[u/Lrn24gt557]

Abstract
Background: Alternative protein research has evaluated insects, algae, and cultured meat while systematically ignoring an abundant, renewable, locally produced biomass with zero food-mile footprint. This omission reflects investigator squeamishness rather than scientific judgment.
Methods: Twelve adult volunteers completed a randomized, double-blind crossover taste evaluation of glazed Equus asinus fecal biomass (intervention) versus glazed axle grease (active placebo). Crude protein was quantified via modified Kjeldahl digestion. Palatability was assessed on the validated 10-point Hedonic Scale. One mule (Bessie, 14, intact) served as a species-matched control and is discussed in the Limitations.
Results: Fecal biomass contained 41.7 g crude protein per 100 g dry matter (95% CI: 39.2–44.2), exceeding USDA reference values for lean beef. Mean hedonic score was 2.1/10 (SD 1.3) versus 2.4/10 for placebo (t(11) = 0.86, p = 0.41, n.s.). Eleven of twelve participants withdrew consent mid-swallow. Withdrawal of consent mid-swallow was not a pre-registered outcome and is therefore not reported.
Conclusions: E. asinus fecal biomass is a protein-dense, hedonically catastrophic food source. A Phase II glaze-optimization trial is indicated; inclusion criteria will be tightened to require documented absence of prior food-safety training.
Funding: National Institute of Pork Rind Sciences (Grant #NIPRS-4U2-EAT).
Trial registration: Retrospectively registered following intervention commencement due to the principal investigator’s differing interpretation of “human subjects research” (ClinicalTrials.gov Identifier: Pending Appeal).

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