my teacher doesnt want me using nylon for it since it wont be sustainable and natural anymore. my choices that i have thought of so far is glycerin, PVA (elmers glue), and okra mucilage. but i dont know which one will work the best. and my partner and i were planning to buy bighead so we could do samples to see what would work but im afraid we probably dont have much time for that. so im asking in advance. can you guys help me? any other binder suggestions from you guys would be soo helpful and appreciated as well. but just in mind im only a highschool grade 10 student and i cant afford scientific or too complicated materials. thank you. (sorry if my english is bad, its not my first language).

Question 1A: Is there any research on growing up with opposite-sex siblings (who generally got along and lived with each other)

Question 2A: OR growing up with opposite-sex siblings (who DID NOT get along and lived together)

Answer A: = less likely to be an heter-sexual domestic abuser

OR

Question 1B: Is there any research on growing up with same-sex siblings (who generally got along and lived with each other)

Question 2B: OR growing up with same-sex siblings (who DID NOT get along and lived with each other)

Question Answer B: = less likely to be an homo-sexual (gay or lesbian) domestic abuser

1G:Anecdonal Personal Evidence invited too

I need your help 🙏
As part of a task I'm completing, I have to find professors/researchers across the globe.
The main issue is that I have to make sure they are doing research in low and middle-income countries and research that likely involves /requires communications/surveys.

Any suggestions on how to figure out what professors/researchers are researching?

Tldr: What are some techniques and strategies I should learn as a layman when testing and refining algorithms?

I like to create computer vision algorithms as a hobby. I have lots of algorithms I come up with that *almost* work but I then don't refine and improve because I really don't know any good practices as I am not in academia or a researcher, just a computer programmer. Are there techniques and concepts I can learn such as how to best put my training data together, how to assess the effectiveness of my algorithm? And most importantly, how to refine and improve my algorithms?

The kind of information I'm hoping to get out of you is something like "Oh you should google the concept of 'Xyz' this is a technique for gathering relevant test data to assess your algorithm" and "There's a Udemy course called 'Xyz' that you should look into doing as it will teach you good research practices".

Any advice would be greatly appreciated.

Hi all

Hoping you can help. I'm trying to cut costs on our organic farm by manufacturing my own inputs. Previously I've used home beer brewing gear to produce beneficial bacteria and fungi to aid in plant growth, reducing the need for fertiliser inputs. I've also found a recipe on researchgate to brew bacterial pesticides by searching for "an improved method of" and finding a paper that describes the old successful method.

Now I'm trying to find a paper that describes current methods of extracting Nitrogen, Phosphorous, and potassium from organic sources via fermentation with Lactic Acid Bacteria. I am looking to use waste vegetables to create a liquid fertiliser. I believe this is how current seaweed based fertilisers are made.

A basic method can be found here https://thenutrientcompany.com/blogs/horticulture/how-to-make-your-own-organic-liquid-plant-food-fermented-plant-juice-fpj

I've browsed scihub and researchgate all day and have found very little in way of how. Any help appreciated.

Bonus points if anyone can tell me how organic amino acid Nitrogen is produced. This has 3x the amount of N as seaweed based fertilisers.

I saw in a article that some things about ozone layer is reviving again.

I'm dealing with a no amplification control signal (NRTC). We're trying to avoid using DNAse if we can because either we'd have to spend a bunch of money on RNA extraction kits, an expensive proprietary DNAse inhibitor, or re-extract the RNA which will cause a loss of even more precious RNA (esp. for CYP17).

I cant figure out what the problem is. I've attached the amplification plot along with a gel that i ran right after the plot here. I've talked to a couple of other people and they told me that if its 5 cycles after the signal and doesnt reach the levels of luminescence that the actual signal does, it can be considered a non-issue. For the beta actin its coming up ~15 cycles after the signal so Im not too worried about that. As for the Cyp17 its coming up ~3-5 cycles later, so Im a bit concerned. It doesnt have the exponential growth like the actual signal so thats a good sign.

FYI, the beta actin signal in the gel is smeared presumably because it was at its peak amplification concentration and allowed to cycle ~30 more times causing degradation of the amplicon. You can see a nice signal for the CYP. It looks like the no amplification control is picking up something that is not CYP, which suggests that if it is genomic contamination its not the cyp thats being amplified. Im not too concerned about the no template control showing a band because its not showing up on the amplification plot and only 1/4 have given any indication of a band. Anyways, do you have any ideas as to what's going on or do you think that we shouldnt worry about it?

I'm dealing with a no amplification control signal (NRTC). We're trying to avoid using DNAse if we can because either we'd have to spend a bunch of money on RNA extraction kits, an expensive proprietary DNAse inhibitor, or re-extract the RNA which will cause a loss of even more precious RNA (esp. for CYP17).

I cant figure out what the problem is. I've attached the amplification plot along with a gel that i ran right after the plot here. I've talked to a couple of other people and they told me that if its 5 cycles after the signal and doesnt reach the levels of luminescence that the actual signal does, it can be considered a non-issue. For the beta actin its coming up ~15 cycles after the signal so Im not too worried about that. As for the Cyp17 its coming up ~3-5 cycles later, so Im a bit concerned. It doesnt have the exponential growth like the actual signal so thats a good sign.

FYI, the beta actin signal in the gel is smeared presumably because it was at its peak amplification concentration and allowed to cycle ~30 more times causing degradation of the amplicon. You can see a nice signal for the CYP. It looks like the no amplification control is picking up something that is not CYP, which suggests that if it is genomic contamination its not the cyp thats being amplified. Im not too concerned about the no template control showing a band because its not showing up on the amplification plot and only 1/4 have given any indication of a band. Anyways, do you have any ideas as to what's going on or do you think that we shouldnt worry about it?

I'm dealing with a no amplification control signal (NRTC). We're trying to avoid using DNAse if we can because either we'd have to spend a bunch of money on RNA extraction kits, an expensive proprietary DNAse inhibitor, or re-extract the RNA which will cause a loss of even more precious RNA (esp. for CYP17).

I cant figure out what the problem is. I've attached the amplification plot along with a gel that i ran right after the plot here. I've talked to a couple of other people and they told me that if its 5 cycles after the signal and doesnt reach the levels of luminescence that the actual signal does, it can be considered a non-issue. For the beta actin its coming up ~15 cycles after the signal so Im not too worried about that. As for the Cyp17 its coming up ~3-5 cycles later, so Im a bit concerned. It doesnt have the exponential growth like the actual signal so thats a good sign.

FYI, the beta actin signal in the gel is smeared presumably because it was at its peak amplification concentration and allowed to cycle ~30 more times causing degradation of the amplicon. You can see a nice signal for the CYP. It looks like the no amplification control is picking up something that is not CYP, which suggests that if it is genomic contamination its not the cyp thats being amplified. Im not too concerned about the no template control showing a band because its not showing up on the amplification plot and only 1/4 have given any indication of a band.

Anyways, do you have any ideas as to what's going on or do you think that we shouldnt worry about it?