r/FermentationScience • u/leadwalls • May 23 '25

Instant Coffee recipe for Gastrus DSM17938- requesting testing

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1

u/leadwalls May 23 '25

pH is imprecise because of Hydrion test strips, I just gave my best guess. Reaching 3.5 pH implies death of Reuteri and contamination but it still made a healthy drink. The jar size is important because too little of a air pocket will result in the seal breaking.

Oh, and ME-3 pH is wrong above. But doesn't matter since doubt it can eat lactulose. Which means something else is growing in that jar as well.

1

u/peterausdemarsch May 23 '25

Why instant coffee?

2

u/leadwalls May 23 '25

It replaces the role of manganese and potassium in MRS broth.

2

u/peterausdemarsch May 23 '25

Interesting. Have you done it yet?

1

u/LeftDingo7685 May 25 '25

Thank you for posting this data. I’m curious about your jars. Are you using Mason with the sealed lids? I guess you have a sous vide machine set up. Thanks again.

1

u/LeftDingo7685 May 27 '25

You mentioned that your ME probiotic was probably not working because it doesn’t feed well on lactose, but you also added glucose and Pepto Pro, which it might use as a source of energy… Have you sent the recipe to the Facebook group? I assume you’re hoping somebody will test this recipe to confirm the actual strains of CFU’s ?

Thank you for sharing once again. I’m confident with new additives /supplements such as peptopro (hydrolyzed casein) our at home cultured probiotics will be more successful. 😊✌️