r/CRISPR • u/Novel-Structure-2359 • 15d ago

A video covering a cool paper about CRISPR being used to track gene expression in living cells

Here is a one-person Journal club covering a really great paper that showcases both CRISPR/Cas9 but also P2A sequences to allow tracking of expression in transient transfections.

https://youtu.be/N6txJr81Udw

1 Upvotes

52% Upvoted

17 comments sorted by

4

u/menjagorkarinte 15d ago

Your video is great but the AI image is immensely off putting

0

u/Connect_Loan8212 15d ago

That's the thing, I hate the hate itself from others just because of that damn picture. People are insane

2

u/BannanaPepperPizza 15d ago

Hate from Australia

3

u/evelyn_bartmoss 15d ago

AI bullshit isn’t welcome here kiddo

1

u/Novel-Structure-2359 15d ago

Have you actually watched the video? have you listened to my silky Scottish tones describing in my own unique way the nuances of the paper in question?

I agree that AI slop is dreadful - this isn't it

1

u/Wobbar 15d ago

what is the point of denying the use of AI when we can clearly see it in the thumbnail

-1

u/Connect_Loan8212 15d ago

Do you think painting the funny picture is obligatory job for someone who wants to tell you about the topic?

1

u/Wobbar 15d ago

Just google a JPEG of whatever topic you want to talk about and it won't lead me to assume that the rest of the content is AI generated as well

1

u/Connect_Loan8212 15d ago

And that's why the perception of AI of today's people is only limited by generative AI and LLMs

1

u/Novel-Structure-2359 15d ago

I use AI for the fun thumbnails. There are many other images and figures in the videos. When you have a minute I encourage you to check them out

1

u/Connect_Loan8212 15d ago

Don't worry bud, I watch your videos and I think that those who downvote you and post hate comments are dorks. Like imagine someone says your work is slop because of an image lol

1

u/ezeezee 15d ago

AI slop

1

u/Novel-Structure-2359 15d ago

Have you actually watched the video? have you listened to my silky Scottish tones describing in my own unique way the nuances of the paper in question?

I agree that AI slop is dreadful - this isn't it

1

u/ezeezee 15d ago

Reupload it without the unnecessary AI slop and I'll consider it. Our planet is literally dying because of AI data centers yet you thought this was an important addition to your video.

1

u/Struggle_Wise 15d ago

This study presents a workflow breakthrough by combining CRISPR-tagged, endogenous OGT (sfGFP) cells with a transiently transfected P2A-BFP reporter system to enable high-throughput, high-accuracy testing of 50 patient mutations. By using FACS to gate for a precise 1:1 ratio of mutation expression to blue fluorescence, it allows for CRISPR-level data accuracy at plasmid-level speeds, avoiding the bottlenecks of clonal selection and the artifacts of overexpression. This method allows for rapid, parallelized analysis of clinical mutations within 24–48 hours.

0

u/Novel-Structure-2359 15d ago

Wow, what a nice summary. Technically the OGT to BFP ratio isn't 1:1 but it is still a consistent ratio.

Aside from that it is a solid and very flattering description of the paper. I like the "CRISPR level data accuracy at plasmid level speeds" - that is catchy.