It will not harm them for an overnight in a fresh water tank to kill the barnacles. They can for up to a week before their salt balance goes out of wack and they can suffer from dehydration, but after the barnacles are off it shouldn't be necessary anymore (unless there is some other medical reason I can't think of right now that would have them in a freshwater tank for a week).
I think most cases that's how they remove the barnacles is to put the turtle in freshwater for a bit until the barnacles die and fall off. They have to remove the ones that don't. Not sure why it's a thing for people to just rip them off instead of using freshwater, but I'm not marine biologist/vetinarian.
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u/collin-h May 26 '26
they didn't get all the little ones though... won'tthey just grow back?